pcr (polymerase chain reaction)
11Nested polymerase chain reaction — A diagram illustrating the method of nested PCR. Nested polymerase chain reaction is a modification of polymerase chain reaction intended to reduce the contamination in products due to the amplification of unexpected primer binding sites.… …
12Digital polymerase chain reaction — (digital PCR, DigitalPCR, dPCR, or dePCR) is a refinement of conventional polymerase chain reaction methods that can be used to directly quantify and clonally amplify nucleic acids including DNA, cDNA or RNA. The key difference between dPCR and… …
13Touchdown polymerase chain reaction — or touchdown style polymerase chain reaction is a method of polymerase chain reaction by which primers will avoid amplifying nonspecific sequence. The temperature at which primers anneal during a cycle of polymerase chain reaction determines the… …
14Inverse polymerase chain reaction — (Inverse PCR) is a variant of the polymerase chain reaction that is used to amplify DNA with only one known sequence. One limitation of conventional PCR is that it requires primers complementary to both termini of the target DNA, but this method… …
15Real-time polymerase chain reaction — In molecular biology, real time polymerase chain reaction, also called quantitative real time polymerase chain reaction (qPCR) or kinetic polymerase chain reaction, is a laboratory technique based on the polymerase chain reaction, which is used… …
16History of polymerase chain reaction — (This article assumes familiarity with the terms and components used in the PCR process.) The history of the Polymerase Chain Reaction (or PCR) has variously been described as a classic Eureka! moment [http://nobelprize.org/nobel… …
17Overlap extension polymerase chain reaction — This page assumes familiarity with the terms and components used in polymerase chain reaction (PCR) process. The overlap extension polymerase chain reaction (or OE PCR) is a variant of PCR. It is also referred to as Splicing by overlap extension… …
18Reverse transcription polymerase chain reaction — In molecular biology, reverse transcription polymerase chain reaction (RT PCR) is a laboratory technique for amplifying a defined piece of a ribonucleic acid (RNA) molecule. The RNA strand is first reverse transcribed into its DNA complement or… …
19Polymerase chain reaction inhibitors — PCR inhibitors usually affect PCR through interaction with DNA or interference with the DNA polymerase. Inhibitors can escape removal during the DNA purification procedure by binding directly to single or double stranded DNA.[1] Alternatively, by …
20Rapid Amplification Of CDNA-ends By Polymerase Chain Reaction — L acronyme de l expression anglaise Rapid amplification of cDNA ends by polymerase chain reaction signifie en français Amplification rapide d extrémités de cDNA par Réaction en chaîne par polymérase . La première partie de l acronyme RACE (… …
