ion exchange chromatography
31Countercurrent exchange — Counter heat current exchange: Note the gradually declining differential and that the once hot and cold streams exit at the reversed temperature difference; the hotter entering stream becomes the exiting cooler stream and vice versa.… …
32Elution — Chromatography column Elution is a term used in analytical and organic chemistry to describe the process of extracting one material from another by washing with a solvent (as in washing of loaded ion exchange resins to remove captured ions). In a …
33Protein purification — is a series of processes intended to isolate a single type of protein from a complex mixture. Protein purification is vital for the characterisation of the function, structure and interactions of the protein of interest. The starting material is… …
34separation and purification — ▪ chemistry Introduction in chemistry, separation of a substance into its components and the removal of impurities. There are a large number of important applications in fields such as medicine and manufacturing. General principles… …
35Conductivity (electrolytic) — The conductivity (or specific conductance) of an electrolyte solution is a measure of its ability to conduct electricity. The SI unit of conductivity is siemens per meter (S/m). Conductivity measurements are used routinely in many industrial and… …
36Temperature-responsive polymer — A temperature responsive polymer is a polymer which undergoes a physical change when external thermal stimuli are presented. The ability to undergo such changes under easily controlled conditions makes this class of polymers fall into the… …
37Phosphoproteomics — is a branch of proteomics that identifies, catalogs, and characterizes proteins containing a phosphate group as a post translational modification. Phosphorylation is a key reversible modification that regulates protein function, subcellular… …
38Protein sequencing — Proteins are found in every cell and are essential to every biological process, protein structure is very complex: determining a protein s structure involves first protein sequencing determining the amino acid sequences of its constituent… …
39Chromatofocusing — is a protein separation technique that allows resolution of single proteins and other ampholytes from a complex mixture according to differences in their isoelectric point. Chromatofocusing utilizes ion exchange resins and is typically performed… …
40Depyrogenation — refers to the removal of pyrogens from solution, most commonly from injectable pharmaceuticals. A pyrogen is defined as any substance that can cause a fever. Bacterial pyrogens include endotoxins and exotoxins, although many pyrogens are… …
