enzyme amplification
1Nicking enzyme amplification reaction — (NEAR) is a method for in vitro DNA amplification like the polymerase chain reaction (PCR). NEAR is isothermal, replicating DNA at a constant temperature using a polymerase (and nicking enzyme) to exponentially amplify the DNA at a temperature… …
2Enzyme-activated MR contrast agents — Molecular imaging is broadly defined as the visualization of molecular and cellular processes on either a macro or microscopic level. Because of its high spatial resolution and ability to noninvasively visualize internal organs, magnetic… …
3Nicking enzyme — A nicking enzyme (or nicking endonuclease) is an enzyme that cuts one strand of a double stranded DNA at a specific recognition nucleotide sequences known as a restriction site. Such enzymes hydrolyse, cut, only one strand of the DNA duplex, to… …
4Multiple displacement amplification — (MDA) is a non PCR based DNA amplification technique. This method can rapidly amplify minute amount of DNA samples to reasonable quantity for genomic analysis. The reaction starts by annealing random hexamer primers to the template and DNA… …
5Helicase-dependent amplification — (HDA) is a method for in vitro DNA amplification like the polymerase chain reaction (PCR). The disadvantage of PCR is that it consumes a lot of time with uncoiling the double stranded DNA with heat into single strands (a process called… …
6Rapid Amplification of cDNA Ends — RACE, or Rapid Amplification of cDNA Ends, is a technique used in molecular biology to obtain the partial sequence of an RNA transcript found within a cell. RACE results in the production of a DNA copy of the RNA sequence of interest, produced… …
7humoral amplification systems — a collective term for the four enzyme cascades that serve as amplification and control mechanisms in hemostasis, inflammation, and tissue repair, including the complement, coagulation, fibrinolytic, and kinin systems …
8Variants of PCR — This page assumes familiarity with the terms and components used in the Polymerase Chain Reaction (PCR) process. The versatility of PCR has led to a large number of variants: Contents 1 Basic modifications 2 Pretreatments and extensions 3 Other… …
9Near — or Near may refer to: Contents 1 Science, mathematics, technology, biology, and medicine 2 Geography 3 Lingu …
10Polymerase chain reaction — PCR redirects here. For other uses, see PCR (disambiguation). A strip of eight PCR tubes, each containing a 100 μl reaction mixture The polymerase chain reaction (PCR) is a scientific technique in molecular biology to amplify a single or a… …
