- dideoxy sequencing
- расшифровка последовательности ДНК
Большой англо-русский и русско-английский словарь. 2001.
dideoxy sequencing
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dideoxy sequencing — (= Sanger dideoxy sequencing) The most popular method of DNA sequence determination (cf. Maxam Gilbert method). Starting with single stranded template DNA, a short complementary primer is annealed, and extended by a DNA polymerase. The reaction… … Dictionary of molecular biology
Sequencing — Learning the order of nucleotides (base sequences) in a DNA or RNA molecule or the order of amino acids in a protein. In the case of DNA sequencing, the precise ordering of the bases (A,T,G,C) from which the DNA is composed is determined. * * *… … Medical dictionary
Microfluidic Sanger sequencing — The completion of the Human Genome Project has been a cornerstone in the advancement of biological studies. The outcomes of obtaining a complete reference map (including the sequence) of the human genome have ushered in the post genome era of… … Wikipedia
DNA sequencing — ▪ genetics technique used to determine the nucleotide sequence of DNA (deoxyribonucleic acid). The nucleotide sequence is the most fundamental level of knowledge of a gene or genome. It is the blueprint that contains the instructions for… … Universalium
Primer (molecular biology) — A primer is a strand of nucleic acid that serves as a starting point for DNA synthesis. They are required for DNA replication because the enzymes that catalyze this process, DNA polymerases, can only add new nucleotides to an existing strand of… … Wikipedia
List of molecular biology topics — This is a list of topics in molecular biology. See also list of biochemistry topics. NOTOC # 3 end 3 flanking region 5 end 5 flanking region 5 ribose 3 A acrylamide gels adenine adenosine deaminase deficiency adenovirus agarose gel… … Wikipedia
Maxam-Gilbert method — A method of DNA sequencing, based on the controlled degradation of a DNA fragment in a set of independent, nucleotide specific reactions. The resulting fragments have characteristic sizes depending on the sequence of the template, that can be… … Dictionary of molecular biology
DNA sequence analysis — Determination of the nucleotide sequence of a length of DNA. Typically, this is performed by cloning the DNA of interest, so that enough can be prepared to allow the sequence to be determined, usually by the Sanger dideoxy sequencing method or… … Dictionary of molecular biology
Two-hybrid screening — Overview of two hybrid assay, checking for interactions between two proteins, called here Bait and Prey . A. Gal4 transcription factor gene produces two domain protein ( BD and AD ) which is essential for transcription of the reporter gene ( LacZ … Wikipedia
Sanger-Coulson method — See dideoxy sequencing … Dictionary of molecular biology
Sequence assembly — In bioinformatics, sequence assembly refers to aligning and merging fragments of a much longer DNA sequence in order to reconstruct the original sequence. This is needed as DNA sequencing technology cannot read whole genomes in one go, but rather … Wikipedia
