supercoiled plasmid
1supercoiled plasmid — The predominant in vivo form of most plasmids, in which the DNA is coiled around histone like proteins. When supporting proteins are stripped away during DNA extraction from the bacterial cell, the plasmid molecule also tends to supercoil around… …
2Plasmid preparation — Plasmid miniprep. 0.8% agarose gel ethidium bromide stained. A plasmid preparation is a method used to extract and purify plasmid DNA. Many methods have been developed to purify plasmid DNA from bacteria. These methods invariably involve three… …
3Plasmid — Figure 1: Illustration of a bacterium with plasmid enclosed showing chromosomal DNA and plasmids. In microbiology and genetics, a plasmid is a DNA molecule that is separate from, and can replicate independently of, the chromosomal DNA …
4Gel electrophoresis of nucleic acids — Digital printout of an agarose gel electrophoresis of cat insert plasmid DNA …
5Agarose gel electrophoresis — is a method used in biochemistry and molecular biology to separate DNA, or RNA molecules by size. This is achieved by moving negatively charged nucleic acid molecules through an agarose matrix with an electric field (electrophoresis). Shorter… …
6Transfection — is the process of introducing nucleic acids into cells by non viral methods [http://www.promega.com/guides/transfxn guide/transfxn.pdf] . The term transformation is preferred to describe non viral DNA transfer in bacteria and non animal… …
7Isopycnic centrifugation — Isopycnic centrifugation, also known as density gradient centrifugation or equilibrium sedimentation is a technique used to separate molecules on the basis of buoyant density. (The word isopycnic means equal density .) Typically, a self… …
8DNA supercoil — Supercoiled structure of circular DNA molecules with low writhe. Note that the helical nature of the DNA duplex is omitted for clarity …
9Restriction map — A restriction map is a map of known restriction sites within a sequence of DNA. Restriction mapping requires the use of restriction enzymes. In molecular biology, restriction maps mdash;along with DNA DNA hybridization, and DNA or RNA sequence… …
10nucleic acid — /nooh klee ik, klay , nyooh /, Biochem. any of a group of long, linear macromolecules, either DNA or various types of RNA, that carry genetic information directing all cellular functions: composed of linked nucleotides. [1890 95; NUCLE(US) + IC;… …
